No, spiders do not scream when killed, because spiders do not have vocal cords, lungs that produce sound like mammals, or ability to make screaming noises, and what people may interpret as scream is usually other sounds like air escaping or imagination. Spiders are arachnids that lack sound producing organs for screaming, though some species can make faint sounds through stridulation, rubbing body parts together, such as some tarantulas that make hissing sound when threatened, but this is not screaming and is rarely heard in house spiders. When spider is squished, any sound heard is typically from exoskeleton cracking or air being expelled, not vocal scream. Some people may hear high pitched noise when killing spider due to air escaping from book lungs, but it is not screaming in sense of vocal pain expression. Spiders do not experience pain in same way mammals do, though they have nervous system that detects damage. So spiders do not scream when killed, and idea of screaming spider is myth often fueled by fear and dramatization. If you need to remove spider, humane relocation is recommended rather than killing, place container over spider, slide paper underneath, and release outdoors away from house, which avoids any distress. Regular cleaning and sealing cracks reduces need to kill spiders, and most house spiders are harmless and beneficial as they eat other pests.
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