The direct opposite of the verb "splice"—which means to join, braid, or fuse two separate ropes, films, cables, or DNA strands together—is to "unsplice," "disconnect," "sever," "separate," "detach," or "untie." In technical contexts such as carpentry, film editing, or molecular biology, any action that breaks apart a unified continuous bond or divides joined components into individual parts acts as the functional antonym.
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