Tempus AI has attracted substantial capital from a wide array of prominent venture capital firms, institutional funds, and corporate investors throughout its growth stages. Early-stage and pre-IPO backers included prominent venture funds like New Enterprise Associates (NEA) and Revolution, alongside major institutional asset managers like T. Rowe Price and Baillie Gifford. Furthermore, major global technology and financial conglomerates such as Google and SoftBank have made significant strategic financial investments in the company.