The Charlotte 49ers maintain several spirited athletic rivalries spanning their conference alignments and geographic region. In football, the developing rivalry with the Old Dominion Monarchs stands out as a prominent annual clash within conference play, alongside historic regional battles against other regional universities. Across basketball and other athletic programs, the 49ers share spirited competitive histories with conference opponents like East Carolina, UAB, and various traditional mid-major foes, fueling intense matchups that draw passionate crowds of students, alumni, and local fans to campus athletic venues every single season.